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用H~3-螺哌啶酮与小牛尾核细胞受体结合的放射受体分析法(RRA)测定大鼠脑和血清中氟哌啶醇的含量。大鼠口服或腹腔注射氟哌啶醇一定时间后,断头,立即取脑,分离并取所需脑区,保存在0.05M冰冷的Tris-HCl(pH7.7)溶液中,以10份冰冷的重蒸馏水制成匀浆液,贮于-20°低温冰箱中备用。血液收集后,于4°离心(10,000×g)10分钟,血浆或血清贮于-20°低温冰箱中。受体制备:冰冻新鲜小牛尾核加10份0.05MTris-HCl(pH7.7)溶液匀浆后,离心(48,000×g)15分钟,上清液弃去,沉淀物以1份Tris-HCl液再悬浮和
The content of haloperidol in rat brain and serum was determined by radioimmunoassay (RRA) using H ~ 3-spiropentone in combination with BMSCs receptors. After oral or intraperitoneal injection of haloperidol for a certain period of time, the rats were decapitated and their brains removed immediately. The brain regions were isolated and stored in 0.05 M ice-cold solution of Tris-HCl (pH 7.7) Of the redistilled water made of homogenate, stored in a -20 ° low temperature refrigerator spare. After blood collection, centrifuge (10,000 x g) for 10 minutes at 4 ° and plasma or serum in a -20 ° refrigerator. Receptor preparation: Frozen fresh Calf caudate nuclei plus 10 parts of 0.05 M Tris-HCl (pH 7.7) solution was homogenized and centrifuged (48,000 x g) for 15 minutes. The supernatant was discarded and the precipitate was washed with 1 part Tris- Resuspend and