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１６只成年杂种犬复制心肌缺血再灌模型。分为地奥心血康治疗组（ＤＡＸＸＫＧ）和生理盐水对照组（ＮＳＣＧ）。差速离心分离心肌细胞质膜，无机磷法测定心肌细胞膜Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶活性，荧光法测定心肌细胞膜与血清脂质过氧化物（ＬＰＯ）含量。结果表明：（１）ＤＡＸＸＫＧＮａ￣＋，Ｋ￣＋－ＡＴＰ酶活性明显高于ＮＳＣＧ（Ｐ＜０．０１）；（２）血清ＬＰＯ含量，随着再灌时间延长，ＮＳＣＧ呈上升趋势，ＤＡＸＸＫＧ略下降，再灌２４０ｍｉｎ两组比较差异显著（Ｐ＜０．０１）；心肌细胞膜ＬＰＯ含量，ＤＡＸＸＫＧ低于ＮＳＣＧ（Ｐ＜０．０５）；（３）心肌细胞膜Ｎａ￣＋，Ｋ￣＋－ＡＴＰ酶活性与ＬＰＯ含量呈明显负相关（ｒ＝－０．８３，Ｐ＜０．０１）。这些结果提示ＤＡＸＸＫ可通过抗脂质过氧化而实现其保护心肌细胞膜的效应。
16 adult mongrel dogs replicate myocardial ischemia reperfusion model. Dioxin into the blood group (DAXXKG) and saline control group (NSCG). The plasma membrane of myocardium was separated by differential centrifugation, and the Na ̄ +, K ̄ +-ATPase activity was measured by inorganic phosphorus method. The content of lipid peroxidation (LPO) in myocardial cell membrane and serum was determined by fluorescence method. The results showed that: (1) DAXXKGNa ̄+, K ̄+-ATPase activity was significantly higher than NSCG (P <0.01); (2) serum LPO content, NSCG showed an upward trend with longer reperfusion time, DAXXKG slightly Decreased, re-irrigation 240min compared the two groups were significantly different (P <0.01); myocardial cell membrane LPO content, DAXXKG was lower than NSCG (P <0.05); (3) myocardial cell membrane Na ~ ~, K ~ +-ATPase Activity was negatively correlated with LPO content (r=-0.83, P<0.01). These results suggest that DAXXK can achieve its effect of protecting myocardial cell membranes through anti-lipid peroxidation.