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目的 为探讨ＨＢｅＡｇ 阴性ＣＨ－ Ｂ 患者血清ＨＢＶ－ ＤＮＡ 定量检测的意义。方法 应用定量ＰＣＲ法对经肝穿活检确诊的５２ 例ＨＢｅＡｇ 阴性，３３ 例ＨＢｅＡｇ 阳性ＣＨ－ Ｂ 血清进行了ＨＢＶ－ ＤＮＡ 含量检测，并将ＨＢｅＡｇ 阳性与阴性患者ＨＢＶ－ ＤＮＡ 含量、ＨＢｅＡｇ 阴性ＨＢＶ－ ＤＮＡ 高水平与低水平患者肝脏病理损害分别进行了比较。结果 ＨＢＶ－ ＤＮＡ 含量ＨＢｅＡｇ 阳性患者显著高于阴性患者，ＨＢｅＡｇ 阴性ＨＢＶ－ ＤＮＡ 高水平患者肝脏病理损害明显重于低水平患者（ 均Ｐ＜ ００５） 。结论 提示血清ＨＢｅＡｇ 阳性；确为判断ＨＢＶ 复制的良好指标； ＨＢｅＡｇ 阴性ＨＢＶ－ ＤＮＡ 高水平者，尤以重视，及时给予抗病毒等治疗实属必要。
Objective To investigate the significance of quantitative detection of serum HBV DNA in patients with HBeAg-negative CH-B. Methods HBV DNA levels in 52 cases of HBeAg-negative and 33 cases of HBeAg-positive CH-B sera diagnosed by liver biopsy were detected by quantitative PCR. HBV-DNA levels in HBeAg-positive and -negative patients, HBeAg-negative HBV-DNA High-level and low-level patients with liver pathological damage were compared. Results The HBeAg positive patients with HBV DNA level were significantly higher than those with negative HBeAg-negative HBV-DNA. The pathological changes of liver in patients with HBeAg-negative HBV-DNA were significantly higher than those in patients with low HBeAg (all P <005). Conclusions suggest that serum HBeAg positive; indeed to determine a good indicator of HBV replication; HBeAg-negative HBV-DNA high level, especially attention, timely treatment such as antiviral is necessary.