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目的建立颅咽管瘤(CP)动物模型,检测移植瘤的微循环结构和瘤细胞分裂、增殖能力。方法收集人脑颅咽管瘤组织,异种异位移植到BALB/C裸小鼠皮下,建立移植瘤。采用免疫组化法以cytokeratin(CK)鉴定肿瘤表型,CD34标记血管内皮细胞。微染色体维持蛋白6(MCM6)检测瘤细胞增殖活性。结果成功建立了10只动物模型。移植瘤组织自身形成微血管,瘤细胞保持了分裂、增殖活性。微血管密度(MVD)、MCM6二者呈正相关(r=0.410,P<0.05)。釉质上皮型(AE)与鳞形乳头瘤型(SP)移植瘤MVD、MCM6比较差异无显著性意义(P>0.05)。结论采用异种异位移植方法可在裸小鼠皮下初步建立人脑颅咽管瘤动物模型。移植瘤细胞可建立新的微循环结构,具备一定的分裂、增殖能力和血管形成活性。
Objective To establish a craniopharyngioma (CP) animal model to detect the microcirculation and tumor cell division and proliferation of tumor xenografts. Methods Human craniopharyngioma tissues were collected and transplanted into BALB / C nude mice subcutaneously to establish xenografts. Immunocytochemistry was used to identify the tumor phenotype with cytokeratin (CK). CD34 labeled vascular endothelial cells. Minichromosome maintenance protein 6 (MCM6) detects tumor cell proliferative activity. Results 10 animal models were successfully established. Transplanted tumor tissue itself formed microvessels, tumor cells maintained the division, proliferation activity. Microvessel density (MVD) and MCM6 were positively correlated (r = 0.410, P <0.05). There was no significant difference between MVD and MCM6 in enamel epithelium (AE) and squamous papillary tumor (SP) (P> 0.05). Conclusion The method of xenotransplantation can be used to establish an animal model of human craniopharyngioma in nude mice subcutaneously. Transplanted tumor cells can establish a new microcirculation structure, with a certain degree of division, proliferation and angiogenic activity.