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在用琼脂瀰散法测定抗菌素活性时,一般常常应用一些芽胞型桿菌作为试验菌,因为它们可以使实验简单化。作者为了得到更进一步加速抗菌素活性测定前的生物学方法,所以对Bsc.subtilis,Bac.mycoides等芽胞菌进行了研究。他们以不同成分含量和pH的培养基,不同的培养温度和时间等研究了芽胞发芽最适条件和影响芽胞发芽速度的各种因素。由实验结果得出培养液中氨基氮的含量较高(140mg％),pH8.0时,芽胞
In the determination of antibiotic activity by the agar diffusion method, some Bacillus cells are generally used as test bacteria because they can simplify the experiment. In order to obtain a biological method before accelerating the determination of antibiotic activity, the authors studied the Bacillus sp. Bs.subtilis and Bac.mycoides. They studied the optimum conditions of sprouting and the various factors that affect the sprouting speed of sprouts with different content and pH of medium, different culture temperature and time. The experimental results showed that the content of amino nitrogen in the culture medium was high (140mg%). When the pH value was 8.0,