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[目的 ]探讨蛋白激酶C在缺血预处理大鼠肾脏缺血再灌注损伤中的作用 .[方法 ]将大鼠随机分组 ,观察大鼠肾脏病理组织学变化 ,用免疫组织化学方法检测不同灌注时间蛋白激酶C水平 .[结果 ]预处理后缺血再灌注组病理组织学肾小管评分均低于缺血再灌注组 ,而预处理后缺血再灌注组和对照组之间无显著性差异 ;预处理后缺血再灌注组蛋白激酶C的表达高于缺血再灌注组 ,并且在再灌注 2h和 6h时 ,预处理后缺血再灌注组中的表达高于对照组 .[结论 ]缺血预处理对大鼠肾脏缺血再灌注损伤有保护作用 .
[Objective] To explore the role of protein kinase C in the renal ischemic reperfusion injury in ischemic preconditioning rats. [Methods] The rats were randomly divided into groups, the pathological changes of the kidneys of the rats were observed, and the different perfusion Time protein kinase C levels. [Results] The histopathological tubule score of ischemic reperfusion group was lower than that of ischemia-reperfusion group after pretreatment, but there was no significant difference between ischemic reperfusion group and control group ; The expression of protein kinase C in ischemic reperfusion group was higher than that in ischemia-reperfusion group after pretreatment, and the expression in ischemic reperfusion group was higher than that in control group at 2h and 6h after reperfusion [Conclusion] Ischemic preconditioning protects renal ischemia reperfusion injury in rats.