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作者对140例女性原发性乳腺癌患者进行研究,观察乳腺癌组织病理与雌激素受体水平间的关系。标本来自乳腺活检,同时进行冰冻切片显微镜检查和雌激素受体蛋白(EKP)的测定。剩余标本接受统一组织学检查,记录肿瘤组织学类型、分化程度、上皮细胞结构、淋巴细胞反应、钙化和坏死。生化方法是:细胞浆内的雌激素受体蛋白,在有或没有冷雌二醇情况下,同氚标记的雌二醇保温,用琼脂电脉测定。每克组织蛋白,肿瘤结合>10fmol 雌二醇,即作为受体阳性。
The authors studied 140 women with primary breast cancer and observed the relationship between histological pathology and estrogen receptor levels. Specimens were derived from breast biopsies, while cryosection microscopy and estrogen receptor protein (EKP) measurements were performed. The remaining specimens were subjected to a uniform histological examination to record the histological type, differentiation, epithelial cell structure, lymphocyte reaction, calcification, and necrosis. The biochemical method is: The cytoplasmic estrogen receptor protein, with or without cold estradiol, is incubated with technetium-labeled estradiol and determined on an agar. Per gram of tissue protein, the tumor binds >10 fmol of estradiol, which is positive as a receptor.