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目的:研究榄香烯对体外培养的人肺腺癌细胞株A549的作用及其机制。方法:采用MTT法检测药物对细胞的抑制作用,流式细胞术检测细胞周期变化以及电镜观察细胞的形态变化。结果:榄香烯能明显抑制肺腺癌细胞A549的生长,其半数生长抑制剂量为124.61μg/ml;流式细胞术证实榄香烯能阻滞肺腺癌细胞从S期进入G2/M期;透射电镜超微结构可见细胞浆内脂滴增多和坏死细胞明显增多的形态变化。结论:榄香烯能抑制肺腺癌细胞的生长,并阻滞肺腺癌细胞从S期进入G2/M期。
Objective: To study the effect and mechanism of elemene on human lung adenocarcinoma cell line A549 cultured in vitro. Methods: The inhibitory effect of drugs on the cells was detected by MTT assay. Cell cycle changes were detected by flow cytometry and cell morphology was observed by electron microscopy. RESULTS: Elemene could significantly inhibit the growth of lung adenocarcinoma cell A549. The inhibitory half-inhibition amount was 124.61 μg/ml. Flow cytometry confirmed that elemene could block lung adenocarcinoma cells from entering S phase into G2/M phase. The ultrastructure of transmission electron microscopy showed that the lipid droplets in the cytoplasm increased and the necrotic cells increased significantly. CONCLUSION: Elemene can inhibit the growth of lung adenocarcinoma cells and block lung adenocarcinoma cells entering G2/M phase from S phase.