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1967年Friedman证明干扰素(IFN)结合至细胞表面是建立抗病毒状态的前提。1979年以来,由于引进高效液相层析、反向高效液相层析、亲和层析等纯化蛋白质的新技术,得到了足够量均一的IFN制品,因此能够进行IFN的放射受体研究,获取该受体的直接证据。本文就IFN受体的结合、纯化及理化特性等作一简介。放射标记IFN与细胞的结合激素受体与其配体一般均有很高亲和力(表观解离常数10~(-9)M左右),每个细胞的结合位点数很少,配体和受体的结合常为可
In 1967, Friedman demonstrated that interferon (IFN) binding to the cell surface is a prerequisite for antiviral status. Since 1979, due to the introduction of new technologies such as high performance liquid chromatography, reverse-phase high performance liquid chromatography, affinity chromatography and the like to purify proteins, a sufficient amount of IFN product has been obtained and thus IFN receptor radioactivity can be studied, Obtain direct evidence of this receptor. This article on the IFN receptor binding, purification and physical and chemical properties of a brief introduction. Radiolabeled IFN and cell binding hormone receptors and their ligands generally have a high affinity (apparent dissociation constant 10 ~ (-9) M or so), the number of binding sites per cell is very small, ligand and receptor The combination of often can