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目的初步探讨髓过氧化物酶(myeloperoxidase,MPO)(-463G/A)基因位点突变与砷中毒皮肤病变的关系。方法于2010年12月在山西省山阴县饮水型砷中毒病区抽取家庭中有砷中毒引起的皮肤病变者共48人作为病例组,以无砷中毒皮肤病变者共45人作为对照组。采集口腔黏膜细胞,提取DNA。采用PCR-限制性片段长度多态性(PCR-RELP)法对MPO基因(-463G/A)位点进行基因分型。结果砷中毒组和对照组MPO(-463G/A)位点GA和GG基因型的分布频率间比较,差异无统计学意义(χ2=0.782,P>0.05)。调整性别、年龄等因素后未发现MPO(-463G/A)位点的多态性与饮水型砷中毒皮肤病变的发病风险有关联(ORadj=1.539,95%CI:0.648～3.655)。结论未发现MPO(-463G/A)位点的多态性与饮水型砷中毒皮肤病变的发病风险存在关联。
Objective To investigate the relationship between myeloperoxidase (- 463G / A) gene mutation and arsenic poisoning skin lesions. Methods In December 2010, a total of 48 skin lesions caused by arsenic poisoning in the family were collected from Shanzhou drinking water type arsenic poisoning area in Shanxi Province. A total of 48 patients were selected as the case group. A total of 45 persons without arsenic poisoning skin lesions were used as the control group. Collect oral mucosal cells and extract DNA. The MPO gene (-463G / A) locus was genotyped by PCR-restriction fragment length polymorphism (PCR-RELP). Results There was no significant difference in frequencies of GA and GG genotypes between MPO (-463G / A) sites in arsenism and control groups (χ2 = 0.782, P> 0.05). There was no association between MPO (-463G / A) polymorphism and the risk of developing arsenic poisoning skin lesions (ORadj = 1.539, 95% CI: 0.648-3.655) after adjustment for sex, age and other factors. Conclusion There was no association between MPO (-463G / A) polymorphism and the risk of developing arsenic poisoning in skin lesions.