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目的探讨高血压病合并主动脉夹层患者基质金属蛋白酶9(MMP9)基因-1562C/T基因多态性与临床表现的关系。方法对高血压病合并主动脉夹层患者及高血压病患者用酚氯仿法提取外周血基因组DNA,PCR限制性片段长度多态性法确定MMP9基因-1562C/T基因型。结果(1)高血压病合并主动脉夹层患者中,MMP9基因-1562T等位基因频率(17.6%)显著高于高血压病患者(11.2%,P<0.05),两组之间3种基因型(-1562CC、-1562CT、-1562TT)分布差异无统计学意义(P>0.05)。(2)与-1562CC基因型相比,基因型为-1562CT/TT的主动脉夹层患者累及升主动脉更多见(OR=2.063,95%CI=0.998～4.264,P=0.049)。结论MMP9基因-1562T多态性与中国汉族人群高血压病并主动脉夹层的发病可能相关,T等位基因可能是高血压病并发主动脉夹层的遗传易感因素之一;携带MMP9基因-1562T等位基因的高血压病合并主动脉夹层患者更多累及升主动脉,影响预后。
Objective To investigate the relationship between the polymorphism of MMP-9 gene -1562C / T and clinical manifestations in patients with hypertension and aortic dissection. Methods Peripheral blood genomic DNA was extracted from patients with essential hypertension complicated with aortic dissection and hypertension by phenol-chloroform method. The -1562C / T genotypes of MMP9 gene were determined by PCR restriction fragment length polymorphism. Results (1) The frequency of -1562T allele of MMP9 gene was significantly higher in hypertensive patients with aortic dissection than in hypertensive patients (11.2%, P <0.05). The genotypes of the three genotypes (-1562CC, -1562CT, -1562TT) was no significant difference (P> 0.05). (2) Compared with -1562CC genotype, patients with aortic dissection with -1562CT / TT had more involvement of ascending aorta (OR = 2.063, 95% CI = 0.998-4.264, P = 0.049). Conclusion The -1562T polymorphism of MMP9 gene may be related to the pathogenesis of hypertension and aortic dissection in Chinese Han population. The T allele may be one of the genetic predisposing factors for hypertensive disease complicated with aortic dissection. The polymorphism of MMP9 gene -1562T Allelic hypertension in patients with aortic dissection more involvement of the ascending aorta, affecting the prognosis.