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目的:探讨寿胎丸对小鼠自然流产模型蜕膜组织细胞因子信号转导抑制因子(SOCS)中SOCS1mRNA,SOCS3mRNA表达的影响。方法:设立小鼠正常妊娠与反复自然流产模型,并将反复自然流产模型小鼠随机分为4组,分别为模型组、寿胎丸低,中,高剂量组,应用原位杂交法检测孕14 d各组蜕膜组织SOCS1mRNA和SOCS3mRNA表达。结果:SOCS1mRNA和SOCS3mRNA在各组均有表达,模型组SOCS1mRNA比正常妊娠组明显升高(P<0.01),而SOCS3mRNA比正常妊娠组明显降低(P<0.01);寿胎丸低,中,高剂量组SOCS1mRNA表达明显低于模型组(P<0.01),而SOCS3mRNA明显高于模型组(P<0.01)。结论:寿胎丸可能通过降低自然流产模型小鼠蜕膜组织SOCS1mRNA表达,而升高SOCS3mRNA表达,从而调控Th向Th2分化,以达到改善妊娠预后的目的。
Objective: To investigate the effect of ShouTaiWan on the expression of SOCS1mRNA and SOCS3mRNA in cytokine signal transduction inhibitory factor (SOCS) in decidua of spontaneous abortion model in mice. METHODS: A normal pregnancy and recurrent spontaneous abortion model was established in mice. The recurrent spontaneous abortion model mice were randomly divided into 4 groups: the model group and the Shoutai Pill low, middle and high dose groups. The in situ hybridization method was used to detect pregnancy. On the 14th day, the expression of SOCS1mRNA and SOCS3mRNA in the decidua tissue of each group. RESULTS: SOCS1 mRNA and SOCS3 mRNA were expressed in all groups. The SOCS1 mRNA level in the model group was significantly higher than that in the normal pregnancy group (P<0.01), while the SOCS3 mRNA level was significantly lower than that in the normal pregnancy group (P<0.01). Shoutai Wan was low, medium and high. The expression of SOCS1 mRNA in the dose group was significantly lower than that in the model group (P<0.01), while the SOCS3 mRNA was significantly higher than that in the model group (P<0.01). Conclusion: ShouTaiWan may decrease the expression of SOCS1mRNA in the decidua tissue of spontaneous abortion mice, increase the expression of SOCS3mRNA and regulate the Th-Th2 differentiation, so as to improve the prognosis of pregnancy.