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选用含有小麦条锈病抗源S2199的杂交组合(3338/14119//S2199)F4/2*陕354F2代519个单株和其F3家系对S2199抗条锈病基因进行遗传分析和分子标记定位。结果表明,来自条锈病抗源S2199的条锈病抗性为显性单基因控制,暂命名该基因为YrS2199。采用BSA法和SSR分子标记分析,筛选到与抗条锈病基因YrS2199连锁的SSR分子标记Xdp269和Xgwm120,连锁距离分别为0.7cM和11.0cM,并将其定位在2BL染色体末端上。这两个分子标记为S2199抗条锈病基因的分子标记辅助选择和抗病基因聚合提供了便利。通过等位性检测和14个条锈菌生理小种分小种鉴定,初步明确了S2199含有的抗条锈病基因可能是Yr5或其等位基因。抗源S2199是一个具有优良农艺性状的材料,为小麦育种提供了一个新的Yr5或其等位基因供体。
Five crosses (F338 / 14119 / S2199) and F2 (F335) of F340 were crossed with the stripe rust resistant S2199 of wheat stripe rust, and their F3 lines were used for genetic analysis and molecular marker mapping of the S2199 stripe rust resistance gene. The results showed that the stripe rust resistance derived from stripe rust resistance S2199 was dominated by a dominant single gene and tentatively named YrS2199. The SSR molecular markers Xdp269 and Xgwm120 linked to the stripe rust resistance gene YrS2199 were screened by BSA and SSR molecular markers. The linkage distances were 0.7 cM and 11.0 cM, respectively, and were mapped on the chromosome 2BL ends. Both of these molecular markers facilitated the molecular marker-assisted selection of S2199 stripe rust resistance and the resistance gene cluster. Through allelic detection and identification of 14 stripe rust species, it was initially determined that the stripe rust resistance gene contained by S2199 may be Yr5 or its allele. Source S2199 is a material with excellent agronomic traits that provides a new Yr5 or allele donor for wheat breeding.