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1.库雷蚊属的淡色库蚊和伊蚊属的朝鲜伊蚊吸流行性乙型脑炎病毒後,在24°—26℃下,逐渐减少,经一定时间,又显增生,其滴度与吸病毒当时无大差异,而在14°—16℃下自第2天起逐渐减少至40天其滴度从未复增。 2.被感染的淡色库蚊和朝鲜伊蚊於14°—16℃下15天後或40天後,再转入24°—26℃,其蚊体内病毒滴度均呈现增殖。 3.被感染的淡色库蚊和朝鲜伊蚊,於24°—26℃下15天後,再转入14°—16℃,其滴度都显示骤降。 4.感染的淡色库蚊与朝鲜伊蚊,在24°—26℃下作叮咬幼鼠的传染试验均获成功。而在14°—16℃下蚊的活动性大减,虽有的蚊虫叮咬了幼鼠,其结果均未能将流行性乙型脑炎病毒传染给幼鼠使其发病。 5.本试验的结果与流行性乙型脑炎流行的季节性的关系已作讨论。
1. Culex pipiens mosquito and Aedes albopictus mosquito inhalation of Japanese encephalitis virus, at 24 ° -26 ° C, gradually decreased, and after a certain period of time, and its proliferation, the titer And no significant difference from the virus at the time, and at 14 ° -16 ℃ since the first two days to gradually reduce to 40 days its titers never increased. Infection of Culex pipiens pallens and Aedes albopictus at 15 ° C-16 ° C or 40 days, and then transferred to 24 ° -26 ° C, the mosquito virus titer showed proliferation. 3. The infected Culex pipiens pallens and Aedes albopictus were resuspended at 14 ° -16 ° C after 15 days at 24 ° -26 ° C. Infection of Culex pipiens pallens and Aedes albopictus, in 24 ° -26 ℃ for bite pups infection test were successful. However, at 14 ° -16 ° C, the activity of mosquitoes was greatly reduced. Although some mosquitoes bite young rats, the results failed to transmit the Japanese encephalitis virus to young rats to make the disease. 5. The results of this trial and the seasonal association of the prevalence of Japanese encephalitis have been discussed.