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目的研究胃癌ｐ５３和ｎｍ２３基因异常表达与胃癌生物学行为及病人预后的关系。方法采用链霉素—生物素（ＳＰ）免疫生化技术行ｐ５３和ｎｍ２３免疫组化染色，研究了２０９例胃癌病人的临床病理资料。结果ｐ５３和ｎｍ２３基因的异常表达率分别为４９．７６％和５７．４２％，ｐ５３及ｎｍ２３异常表达与病人性别、年龄、肿瘤部位、大小无关。随着ＴＮＭ分期的进展ｐ５３基因异常表达率升高（Ｐ＝０．００９），Ｂｏｒｍａｎｎ４胃癌ｎｍ２３异常表达率高（Ｐ＝０．０２６），ｐ５３在粘液腺癌低表达（Ｐ＝０．０１８６），ｐ５３及ｎｍ２３与胃癌的淋巴结转移有关（Ｐ＝０．０１８，Ｐ＝０．００２）。ｐ５３及ｎｍ２３与术后生存时间有关。结论ｐ５３及ｎｍ２３基因异常表达能反映胃癌的生物学行为，并与胃癌的预后有关。ｎｍ２３更能提示胃癌转移，但ｐ５３提示预后的能力比ｎｍ２３强。
Objective To study the relationship between the abnormal expression of p53 and nm23 genes and the biological behavior and prognosis of gastric cancer. Methods Streptomycin-biotin (SP) immunohistochemistry was used to perform immunohistochemical staining for p53 and nm23. The clinical and pathological data of 209 patients with gastric cancer were studied. Results The abnormal expression rates of p53 and nm23 genes were 49.76% and 57.42%, respectively. The abnormal expression of p53 and nm23 was not related to the patient’s gender, age, tumor location, and size. With the progression of TNM staging, the abnormal expression rate of p53 gene was increased (P=0.009). The abnormal expression rate of nm23 in Bormann4 gastric cancer was high (P=0.026), and p53 was lowly expressed in mucinous adenocarcinoma (P=0.0186). , p53 and nm23 were associated with lymph node metastasis in gastric cancer (P=0.018, P=0.002). P53 and nm23 are associated with postoperative survival time. Conclusion The abnormal expression of p53 and nm23 genes can reflect the biological behavior of gastric cancer and is related to the prognosis of gastric cancer. Nm23 is more suggestive of gastric cancer metastasis, but p53 is more prone to prognosis than nm23.