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目的 :研究Rapamune +环孢素A(CsA) +强的松 (Pred)和CsA +霉酚酸酯 (MMF) +Pred两种免疫抑制方案对肾移植受者外周血淋巴细胞CD 3、CD 4、CD 8、CD 4 /CD 8、CD 2 5、CD 2 8、CD 95、HLA DR表达动态变化的不同影响。 方法 :以上海交通大学附属第一人民医院器官移植中心 2 0 0 0 10 31至 2 0 0 1 0 8 31所有使用CsA +MMF+Pred或Rapamune +CsA +Pred免疫抑制方案的肾移植受者为研究对象。于肾移植术前、术后 7～ 10天、术后 1个月、术后 3个月检测肾移植受者外周血淋巴细胞CD 3、CD 4、CD 8、CD 4 /CD 8、CD 2 5、CD 2 8、CD 95、HLA DR表达的动态变化。应用SPSS10 0统计软件包分析不同免疫抑制方案对淋巴细胞表面分子表达的影响。 结果 :使用Rapamune +CsA +Pred免疫抑制方案的肾移植受者肾移植术后外周血淋巴细胞CD 3、CD 4、CD 8、CD 2 8的表达较使用CsA +MMF +Pred的受者高。 结论 :使用Rapamune +CsA +Pred的肾移植受者和使用CsA +MMF +Pred的肾移植受者相比较 ,对外周血淋巴细胞CD 3、CD 4、CD 8、CD 2 8表达的影响较小 ,在使用流式细胞仪动态监测肾移植受者免疫状况时应予以考虑
Objective: To investigate the effects of Rapamune + cyclosporine A (CsA + prednisone) and CsA + mycophenolate mofetil (Prednisolone plus Pred + Pred) on peripheral blood lymphocyte CD 3, CD 4 , CD 8, CD 4 / CD 8, CD 2 5, CD 2 8, CD 95, HLA DR expression. METHODS: All kidney transplant recipients who received CsA + MMF + Pred or Rapamune + CsA + Pred immunosuppressive regimen from the Organ Transplantation Center of Shanghai First People’s Hospital, Shanghai Jiaotong University, from 2000 to 2010, were enrolled as Research object The levels of CD 3, CD 4, CD 8, CD 4 / CD 8 and CD 2 in peripheral blood lymphocytes of renal allograft recipients were measured before renal transplantation, 7-10 days after operation, 1 month after operation and 3 months after operation. 5, CD 2 8, CD 95, HLA DR expression. The effects of different immunosuppressive regimens on the expression of lymphocyte surface molecules were analyzed using SPSS10 0 statistical package. RESULTS: Renal transplant recipients using the Rapamune + CsA + Pred immunosuppression regimen showed higher expression of CD 3, CD 4, CD 8 and CD 2 8 in peripheral blood lymphocytes than those receiving CsA + MMF + Pred after kidney transplantation. CONCLUSION: Renal transplant recipients using Rapamune + CsA + Pred have less effect on CD3, CD 4, CD 8, CD 8 expression in peripheral blood lymphocytes compared with those receiving CsA + MMF + Pred , Should be considered when using flow cytometry to dynamically monitor the immune status of renal transplant recipients