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报道一种可全自动、顺序实现样品中杂质颗粒介电过滤和目标颗粒电阻脉冲检测、计数和尺寸判定的微流控芯片装置.根据杂质颗粒与目标颗粒尺寸和介电特性的不同,在芯片通道上顺序设计了介电泳(DEP)分离区和电阻脉冲(RPS)检测区,通过理论模拟,优化并确定芯片结构和尺寸,并进行实验验证,实现了3μm聚苯乙烯杂质颗粒的介电过滤以及近头状伪蹄形藻的检测和计数.研究表明:颗粒收集通道的位置布置对于颗粒的收集效果有重要影响;检测到的近头状伪蹄形藻的RPS信号信噪比(>20)较高,即DEP分离用的高压电源并没有明显影响RPS信号的信噪比.本研究对于发展成分复杂的单细胞生物样品快速检测和分析的微流控芯片便携式设备具有一定的借鉴价值.
Reported a kind of microfluidic chip device that can automatically and sequentially realize impurity particle dielectric filtration and target particle resistance pulse detection, counting and size determination in a sample.According to the difference of impurity particle size and target particle size and dielectric property, The DEP isolation zone and the RPS detection zone are sequentially designed on the channel, and the structure and size of the chip are optimized and verified through theoretical simulation, and the dielectric filtration of 3 μm polystyrene impurity particles is achieved And the detection and counting of near-head-shaped Pseudomonas algae.The results show that the location of the particle collection channel has an important influence on the collection effect of the particles. The signal-to-noise ratio (> 20) ), That is, the high-voltage power supply for DEP separation did not significantly affect the signal-to-noise ratio of RPS signal.This study is of some reference value for the development of microfluidic chip portable devices for the rapid detection and analysis of complex single-cell biological samples.