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为了阐明不同BT型杂交粳稻组合间育性差异的原因,利用豫粳6号A、9201A及其相应保持系与恢复系TR2604、闵120来配制遗传组合就BT型不育细胞质育性恢复遗传进行研究。根据9201A//9201B/闵120、9201A//9201B/TR2604及豫粳6号A//豫粳6号B/闵120三个群体中植株的育性分离,推断9201A、豫粳6号A的育性恢复均由1对恢复基因控制。基因定位结果表明,恢复系闵120中的恢复基因位于第10染色体上18772374-18967996bp间约195kb物理区间内,TR2604中的恢复基因位于第10染色体18 817 631-18 967 996bp间约150kb物理区间内。根据已克隆Rf1基因的信息可知两恢复系带有的恢复基因均为Rf1a。豫粳6号A/TR2604//TR2604BC1F2群体中植株的Rf1a位点基因型与育性分析结果表明TR2604对豫粳6号A育性恢复由Rf1a控制,豫粳6号A/TR2604不育由双亲间的不亲和性造成。
In order to elucidate the reasons for the differences in fertility among different BT-type japonica hybrids, the fertility restoration of BT-typed sterile cytoplasm was carried out using Yujing 6 A, 9201A and their corresponding maintainer and restorer lines TR2604 and Min 120 to make genetic combinations the study. Based on the fertility segregation of 9201A // 9201B / Min 120,9201A // 9201B / TR2604 and Yujing 6 A // Yujing 6 B / Min 120, it was concluded that 9201A, Yujing 6 A Fertility restoration by a pair of restorer gene control. The results of gene mapping showed that the restorer gene in Min 120 was located in the 195kb region between 18772374-18967996bp on chromosome 10 and the restorer gene in TR2604 was located in the region of 150kb between 18 817 631-18 967 996bp on chromosome 10 . According to the information of the cloned Rf1 gene, it can be seen that both restorer lines have the restorer gene Rf1a. The genotype and fertility analysis of Rf1a loci in Yujing 6 A / TR2604 / TR2604BC1F2 population showed that TR2604 was controlled by Rf1a on the fertility of Yujing 6 A, and that of Yujing 6 A / TR2604 from parents Inconsistency caused.