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通过对澳洲青苹茎尖培养及植株再生体系中无菌培养物的建立、初代培养、继代培养、生根培养和移栽五个技术环节的研究,建立了澳洲青苹组织培养快速繁殖技术体系.结果表明: 1 BA0.3～0.6mg/L+NAA0.1～0.3mg/L的MS培养基能促进澳洲青苹茎尖生长或大量侧芽的分化,不定芽生长健壮; 2 无根幼苗在含有IBA0.6～1.0mg/L+NAA0.1mg/L的1/2MS培养基上暗培养一周后,再进行自然光培养,30d生根率可达到78%以上; 3 生根苗经50mg/LNAA浸渍1～2h后,移入蛭石∶腐殖土∶田园土=2∶1∶1的移栽基质中,保温保湿,成活率达80%以上; 4 进一步建立了自然光下生根、练苗一次完成的同步化体系,缩短了繁育时间,简化了步骤,降低了成本.此技术体系为澳洲青苹优良种苗快速繁殖提供了一条新途径.
Through the research on the establishment of the aseptic culture, primary culture, subculture, rooting culture and transplanting in the tip culture and plant regeneration system of P. cantonensis in Australia, the rapid culture technology system The results showed that: 1 BA0.3 ~ 0.6mg / L + NAA0.1 ~ 0.3mg / L of MS medium can promote the growth of shoot tip or a large number of lateral bud differentiation of A. purpurea, adventitious buds grow robust; 2 rootless seedlings in One week culture with 1 / 2MS medium containing IBA0.6 ~ 1.0mg / L + NAA0.1mg / L followed by natural light culture, the rooting rate reached more than 78% after 30 days; 3 rooting seedlings were immersed in 50mg / L NAA 1 ~ 2h later, transferred into the vermiculite: humus soil: garden soil = 2: 1: 1 transplanting matrix, heat moisturizing, the survival rate of 80% or more; 4 to further establish a natural light roots, The system has shortened the breeding time, simplified the steps and reduced the cost, which provides a new way for the rapid propagation of the fine seedlings of P. chinensis.