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应用~(32)P标记的3.2kb克隆HBVDNA探针对36例原发型肝癌患者的血清和肝组织作HBVDNA斑点杂交,结果:血清、肝癌组织、癌近邻肝组织和癌远邻肝组织的阳性率分别为56％、91％88％和80％,皆高于国内外一些同类研究,说明其HBV感染率比一般认为的还要常见,尽管肝内良恶组织的HBV DNA阳性率未见显著差别。此外,尚对HBV6项血清标记作了检测,结果HBsAg和PHSA·Re的阳性率均较高,分别为83％和75％,说明多数病例的HBV感染仍然处于病毒活动复制阶段。由于血清HBsAb阳性者,肝内HBV DNA全阳性,提示HCC阶段的“保护性抗体”并不能清除病毒感染。
Using 32kb P-labeled 3.2kb cloned HBV DNA probes for HBV DNA dot hybridization in serum and liver tissue of 36 patients with primary hepatocellular carcinoma. Results: serum, hepatocellular carcinoma, adjacent adjacent liver tissue and adjacent liver tissue The positive rates were 56%, 91%, 88%, and 80%, respectively, which were higher than some similar studies at home and abroad, indicating that the HBV infection rate was more common than generally thought. Although the positive rate of HBV DNA in benign and malignant tissues in the liver was not seen Significant difference. In addition, six serum markers of HBV were also tested. As a result, the positive rates of HBsAg and PHSA·Re were both high, 83% and 75%, respectively, indicating that HBV infection in most cases is still in the stage of viral replication. Because of the positive serum HBsAb, all liver HBV DNA is positive, suggesting that the “protective antibody” at the HCC stage does not remove the virus infection.