论文部分内容阅读
建立严重急性呼吸综合征(SARS)冠状病毒分离、培养方法,为SARS冠状病毒动物模型的建立提供实验依据,并根据病毒在体内存活的时间确定检测指标。选用已鉴定为SARS冠状病毒的毒株,经过鼻腔接种感染恒河猴。定期采集咽拭子标本,分离血清或血浆,用Vero细胞进行病毒培养、分离。结果显示,在SARS冠状病毒感染恒河猴后2、5、7天,可以从拭子中分离到病毒,5～15天可在猴肺、脾、肝、肾和淋巴组织中分离到病毒,并用免疫荧光法和RT PCR方法进行了确定。首次实验证实了SARS冠状病毒可在恒河猴体内复制。SARS病毒的成功分离是SARS冠状病毒动物模型建立的主要依据,在进行疫苗安全性和药效评价等工作中,病毒分离可作为药物筛选、疫苗评价的重要指标。
To establish a method to isolate and culture coronavirus from severe acute respiratory syndrome (SARS) coronavirus, and to provide an experimental basis for the establishment of SARS coronavirus animal model and to determine the detection index according to the virus’s survival time in vivo. Rhesus monkeys were infected by nasal inoculation using strains that have been identified as SARS coronavirus. Throat swab specimens were collected on a regular basis, serum or plasma was isolated, and the virus was cultured in Vero cells and isolated. The results showed that the virus could be isolated from swabs at 2, 5 and 7 days after infection with rhesus macaques by SARS-Covirus, and the viruses were isolated from the lung, spleen, liver, kidney and lymphoid tissues of monkey in 5-15 days, And confirmed by immunofluorescence and RT PCR methods. The first experiments confirmed that the SARS coronavirus can replicate in rhesus monkeys. The successful isolation of SARS virus is the main basis for the establishment of animal model of SARS coronavirus. In the work of vaccine safety and efficacy evaluation, virus isolation can be used as an important index for drug screening and vaccine evaluation.