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目的研究临床分离的肺炎克雷伯菌对氨基糖苷类抗生素庆大霉素的耐药性与其产铁载体的关系。方法采用K-B纸片法和肉汤稀释法确定70株临床分离的肺炎克雷伯菌对庆大霉素的药物敏感性;CAS琼脂实验检测肺炎克雷伯菌是否产铁载体;紫外可见分光光度法确定细菌产铁载体的量,根据中位数法将70株临床分离菌分为铁载体高产组(35株)和低产组(35株);应用SPSS统计学软件分析抗生素耐药性与其产铁载体是否相关。结果药物敏感性试验检测出菌株对庆大霉素的耐药率为50.00%(35/70);铁载体检测实验确定70株肺炎克雷伯菌均产生铁载体,肺炎克雷伯菌对庆大霉素的耐药性与铁载体产量呈正相关关系(r=0.3154,P<0.05),对庆大霉素耐药菌株铁载体产量明显高于敏感菌株(t=3.1650,P<0.05),且铁载体高产组耐药率及lgMIC值明显高于低产组(x~2=9.6570,t=3.1360,P<0.05)。结论 70株临床分离的肺炎克雷伯菌均产生铁载体,铁载体可能参与肺炎克雷伯菌对庆大霉素的耐药,干扰庆大霉素的抑菌或杀菌过程。
Objective To study the relationship between clinical isolates of Klebsiella pneumoniae against aminoglycoside antibiotics gentamicin and its iron-producing carrier. Methods The drug susceptibility of 70 clinically isolated Klebsiella pneumoniae to gentamicin was determined by KB disk method and broth dilution method. CAS agar assay was used to detect whether Klebsiella pneumoniae produced iron carrier. The UV-Vis spectrophotometry 70 strains of clinical isolates were divided into high-yield group (35 strains) and low-yield group (35 strains) according to the median method. SPSS statistical software was used to analyze the relationship between antibiotic resistance and its production Iron carrier is related. Results The resistance rate of the strain to gentamycin was 50.00% (35/70) in drug susceptibility test. Iron carrier was detected in 70 iron-producing Klebsiella pneumoniae isolates. Klebsiella pneumoniae The drug resistance of gentamicin was positively correlated with the yield of iron carrier (r = 0.3154, P <0.05). The yield of iron carrier of gentamycin resistant strain was significantly higher than that of the susceptible strain (t = 3.1650, P <0.05) The resistance rate and lgMIC value of high-yielding iron-bearing group were significantly higher than those of low-yielding group (x ~ 2 = 9.6570, t = 3.1360, P <0.05). Conclusion 70 strains of Klebsiella pneumoniae isolated from clinical isolates all produce iron carriers, and iron carriers may be involved in the resistance of Klebsiella pneumonia to gentamicin and interfere with the antibacterial or bactericidal activity of gentamicin.