论文部分内容阅读
[目的 ]探讨日本血吸虫虫卵可溶性抗原 (SEA) 38k Da分子诊断血吸虫病的应用价值。 [方法 ]用 SDS-PAGE配合电渗洗脱技术分离纯化了诊断靶抗原 SEA38k Da,并经 SDS- PAGE和 Western blot鉴定 ;将 SEA38k Da分子和 SEA用于 EL ISA法检测日本血吸虫病患者血清 ,急性期 31例、慢性期 31例 ,正常人 6 0例 ,华支睾吸虫病患者血清 30例 ,并殖吸虫病患者血清 30例。 [结果 ]SEA38k Da分子抗原和 SEA所测得的阳性率分别为90 .3%、90 .3%、1.7%、0、0和 90 .3%、83.9%、3.3%、0、0。将 SEA 38k Da分子和 SEA在 EL ISA中的 P/N比值进行比较 ,经 t检验 ,发现在检测急性血吸虫病患者 ,慢性血吸虫病患者和正常人血清时 ,两者间的 P/N比值在 3组间差异均有显著性意义 (P值均 <0 .0 0 1) ;SEA 38k Da分子在检测患者血清时的 P/N比值显著高于 SEA,而检测正常人血清时的 P/N比值显著低于 SEA。 [结论 ]纯化 SEA38k Da具有较好的敏感性和特异性 ,具有诊断潜能
[Objective] To investigate the diagnostic value of 38 kDa molecule of Schistosoma japonicum egg-soluble antigen (SEA) in the diagnosis of schistosomiasis. [Method] The diagnostic target antigen SEA38k Da was isolated and purified by SDS-PAGE combined with electroextraction and identified by SDS-PAGE and Western blot. The SEA38k Da molecule and SEA were used to detect the serum of patients with schistosomiasis japonica by ELISA, There were 31 cases of acute phase, 31 cases of chronic phase, 60 cases of normal, 30 cases of clonorchiasis serum and 30 cases of paragonimiasis serum. [Results] The positive rates of SEA38k Da molecular antigen and SEA were 90.3%, 90.3%, 1.7%, 0,0 and 90.3%, 83.9%, 3.3% and 0,0, respectively. The P / N ratio of SEA 38k Da molecule and SEA in EL ISA was compared. The t-test showed that the P / N ratio between acute schistosomiasis patients, chronic schistosomiasis patients and normal human serum was The P / N ratio of SEA 38k Da molecule in detecting serum was significantly higher than that of SEA, while P / N in normal human serum The ratio was significantly lower than SEA. [Conclusion] The purification of SEA38k Da has good sensitivity and specificity, with diagnostic potential