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目的探讨重组腺病毒联合X射线对U251脑胶质瘤细胞的杀伤作用。方法实验分6组:空白对照组(A组),Ad-Egr-hTrail组(B组),Ad-CMV-hTrail组(C组),单独照射组(D组),Ad-Egr-hTrail+照射组(E组),Ad-CMV-hTrail+照射组(F组)。取对数生长期的U251细胞接种于96孔细胞培养板中,48h后重组腺病毒感染U25细胞,感染2d后分别给予D、E、F组12GyX射线一次性照射,照射后3d,MTS法测定细胞抑制率。结果①B组与A组相比差异无统计学意义(P>0.05),其余各实验组与对照组相比差异有统计学意义(P<0.01)。②两种腺病毒重组载体单独使用时,C组的抑制率明显高于B组(P<0.01),两者联合放疗时,抑制率均明显提高(P<0.01),E组是B组的7.43倍,F组是C组的1.84倍。③与D组相比,E组、F组的抑制率明显提高(P<0.01),E组的放疗增敏比为1.38倍,F组的放疗增敏比为1.53倍。结论①Ad-Egr-hTrail具有很强的辐射诱导特性。②TRAIL基因具有辐射增敏作用。③两种腺病毒重组载体与放射治疗有正协同增敏作用.
Objective To investigate the killing effect of recombinant adenovirus combined with X-ray on U251 glioma cells. Methods The experiment was divided into 6 groups: control group (group A), Ad-Egr-hTrail group (group B), Ad-CMV-hTrail group Group (group E), Ad-CMV-hTrail + group (group F). U251 cells in logarithmic growth phase were inoculated into 96-well cell culture plates and infected with U25 cells after 48h. After infection for 2 days, they were given 12Gy X-rays once, respectively. After 3 days of irradiation, MTS assay Cell inhibition rate. Results ① There was no significant difference between group B and group A (P> 0.05), while there was significant difference between the other experimental groups and control group (P <0.01). ② The inhibitory rates of C and C groups were significantly higher than those of B group (P <0.01) when the two recombinant adenoviruses were used alone (P <0.01) 7.43 times, F group is 1.84 times of C group. ③ Compared with group D, the inhibitory rates of group E and group F were significantly increased (P <0.01). The sensitivity of group E was 1.38 times and that of group F was 1.53 times. Conclusion ① Ad-Egr-hTrail has strong radiation-induced characteristics. ②TRAIL gene has a radiation sensitizing effect. ③ adenovirus recombinant vector and radiotherapy have positive synergistic sensitization.