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为提高寄生虫学实验课的教学质量,让学生识别寄生虫发育各阶段的形态,对于其了解寄生虫的生活史,有着重要的意义.作者近20年从事日本血吸虫胞蚴、尾蚴的永久标本的制作,采用亲水性玻璃纸处理胞蚴,醋酸明矾卡红和梅氏卡红染色作了研究,取得满意效果,现介绍如下.材料与方法一、材料准备 阳性钉螺,亲水性玻璃纸,醋酸明矾卡红、梅氏卡红按文献配制.二、胞蚴制片(一)采用钉螺压碎法,轻压钉螺,取其软组织于镜下撕洗分离后,剪一小块约15×8mm大小的亲水性玻璃纸,将洗净的胞蚴放置于玻璃纸上,然后对折轻压(尽量把胞蚴置于对折后的玻璃纸中央),用盖片压住玻璃纸,湿润固定于
In order to improve the teaching quality of the experimental group of parasitology and let the students identify the morphogenesis of each stage of the parasite development, it is of great significance to understand the life history of the parasite.For nearly 20 years, the permanent specimens of Schistosoma japonicum, cercariae Of the preparation, the use of hydrophilic cellophane treatment of cercariae, alum acetate red and Mylar card red staining were studied and achieved satisfactory results, are described below.Material and methods First, the material Preparation Positive snails, hydrophilic cellophane, acetic acid Alum card red, merck card red prepared according to the literature. Second, the production of cercariae (a) using snail crushing method, light pressure snails, take the soft tissue in the mirror tear separation, cut a small piece of about 15 × 8mm The size of the hydrophilic cellophane, the clean sarcocysis placed on cellophane, and then folded slightly pressure (as far as possible to the double fold after the cellophane placed in the center of the cellophane), with a cover pressed cellophane, wet fixed to