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目的:探讨清纤方抗肝纤维化的治疗机制。方法:利用CCl4制备肝纤维化大鼠模型,分为正常组、模型组、清纤方组、秋水仙碱组。治疗8周后,用HE和Masson染色观察肝纤维化的形成,免疫组化和RT-PCR检测肝组织中TGF-1βmRNA和蛋白的表达。结果:TGF-β1蛋白和mRNA的表达在治疗组均明显减少。结论:清纤方能有效减轻肝纤维化大鼠的肝脏损伤及纤维化程度,其机制可能与抑制肝内TGF-1β的表达有关。
Objective: To explore the treatment mechanism of Qingxian Fang on liver fibrosis. METHODS: Rat models of hepatic fibrosis were prepared with CCl4 and divided into normal group, model group, Qingxian group and colchicine group. After 8 weeks of treatment, HE and Masson staining were used to observe the formation of hepatic fibrosis. Immunohistochemistry and RT-PCR were used to detect the expression of TGF-β mRNA and protein in liver tissue. Results: The expression of TGF-β1 protein and mRNA was significantly reduced in the treatment group. Conclusion: Qingxian Fang can effectively reduce liver damage and fibrosis in rats with hepatic fibrosis. The mechanism may be related to the inhibition of hepatic TGF-1β expression.