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目的:研究宁波市5对HIV感染夫妻的毒株亚型分布特点和基因变异特点。方法:收集宁波市5对已经被确认为HIV-1型阳性夫妻的全血样品,对CD4+T淋巴细胞进行绝对计数,运用套式PCR方法扩增病毒膜蛋白env基因区并进行序列测定。应用DNASTAR软件对序列和参考序列进行比对分析,确定基因亚型。结果:成功获得10条基因序列,确定4株为B′亚型,2株为07_BC流行重组型,4株为08_BC流行重组型。该10例HIV-1阳性全血的CD4+T淋巴细胞绝对数最低133个/μl,最高411个/μl,平均289个/μl。结论:宁波市夫妻感染HIV-1毒株存在多种亚型,大多数的感染者免疫状况低下需要抗病毒治疗,流行形势严峻,应加强对该人群免疫状况和HIV-1毒株亚型变异的监测,及时调整防治策略。
Objective: To study the distribution characteristics and genetic variation of five strains of HIV infected couples in Ningbo City. Methods: Five whole blood samples of HIV-1 positive couples were collected from Ningbo City. Absolute counts of CD4 + T lymphocytes were collected. The env gene of viral membrane protein was amplified by nested PCR and sequenced. DNASTAR software was used to compare the sequences and reference sequences to determine the genotype. Results: Ten gene sequences were successfully obtained, 4 strains were identified as B ’subtype, 2 strains were 07_BC epidemic recombinant and 4 strains were 08_BC epidemic recombinant. The absolute number of CD4 + T lymphocytes in the 10 cases of HIV-1 positive whole blood was at least 133 / μl, up to 411 / μl with an average of 289 / μl. Conclusion: There are many subtypes of HIV-1 strains infected by husband and wife in Ningbo City. Most of the infected patients need anti-virus treatment because of their low immunity status. The epidemic situation is severe. The immune status and subtypes variation of HIV-1 strain should be strengthened Monitoring, timely adjustment of prevention and control strategies.