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目的探讨不同含量碳水化合物饮食对大鼠心脏和脑组织18F-FDG摄取的影响。方法以标准饮食饲养2周后,保持饮食总能量不变,将15只Wistar大鼠随机分为3组:低碳水化合物组(LC)、中碳水化合物组(IC)和高碳水化合物组(HC);每组大鼠每种饮食饲养4周后,对动物行18F-FDGPET全身显像,于第2天尾静脉取血测定酮体、葡萄糖、胰岛素和胰高血糖素的含量。图像经计算机处理,获取心脏和脑的最大标准摄取值(SUVmax)。结果心脏SUVmax分别为:LC组3.27±0.43,IC组10.92±0.89,HC组10.95±0.81;脑SUVmax:LC组4.78±0.19,IC组4.04±0.15,HC组3.77±0.24。LC组与IC和HC组之间的差异均有统计学意义(P<0.001),IC和HC组间差异无统计学意义。LC组酮体为(726.10±65.95)μmol/L,约为IC和HC组的6～7倍;血清酮体水平与心脏SUVmax呈负相关(r=-0.764,P<0.001),与脑SUVmax呈正相关(r=0.569,P<0.001);各组间血清胰岛素、血糖和胰高血糖素的差异均无统计学意义。结论长期极低碳水化合物饮食使大鼠心脏对FDG的摄取明显降低,而脑组织对葡萄糖的摄取略有增加。
Objective To investigate the effects of different carbohydrate diets on uptake of 18F-FDG in rat heart and brain. Methods Fifteen Wistar rats were randomly divided into three groups: low carbohydrate group (LC), middle carbohydrate group (IC) and high carbohydrate group (HC) ); After each diet for 4 weeks in each group, 18 F-FDG PET images of animals were taken and the contents of ketone body, glucose, insulin and glucagon were determined by tail blood sampling on the second day. The images were computerized to obtain the maximum standard uptake value (SUVmax) of the heart and brain. Results SUVmax were 3.27 ± 0.43 in LC group, 10.92 ± 0.89 in IC group and 10.95 ± 0.81 in HC group, respectively. Brain SUVmax was 4.78 ± 0.19 in LC group, 4.04 ± 0.15 in IC group and 3.77 ± 0.24 in HC group. The differences between LC group and IC and HC groups were statistically significant (P <0.001). There was no significant difference between IC and HC groups. The ketone body in LC group was (726.10 ± 65.95) μmol / L, which was about 6 to 7 times higher than that in IC and HC group. Serum ketone level was negatively correlated with SUVmax (r = -0.764, P <0.001) (R = 0.569, P <0.001). There was no significant difference in serum insulin, blood glucose and glucagon among the three groups. Conclusion Long-term, very low carbohydrate diet significantly decreased FDG uptake in the rat heart, while glucose uptake slightly increased in the brain tissue.