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采用一种新颖的方法将(3 巯基丙基) 三甲氧基硅烷凝胶溶胶、纳米金和葡萄糖氧化酶自组装于金电极表面,制得了 高灵敏度的葡萄糖生物传感器.实验表明固定在三维网状的凝胶溶胶中的葡萄糖氧化酶和纳米金颗粒具有很强的稳定性并 且能够保持自身的活性.本实验采用Co(bpy)3+3作为电子媒介体,峰电流值与葡萄糖浓度在8.0×10-10～4.2×10-8mol/L 成线形关系,检测下线为3.0×10-10mol/L(S/N=3).
A novel method of self-assembly of (3-mercaptopropyl) trimethoxysilane gel, nanogold and glucose oxidase on the surface of gold electrode resulted in a high-sensitivity glucose biosensor. Experiments showed that the immobilized glucose Gel electrophoresis of glucose oxidase and gold nanoparticles has a strong stability and can maintain its own activity.In this experiment, the use of Co (bpy) 3 +3 as the electron mediator, the peak current value and the glucose concentration of 8.0 × 10-10 ~ 4.2 × 10-8mol / L has a linear relationship, the detection of off-line 3.0 × 10-10mol / L (S / N = 3).