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目的探讨T细胞疫苗(TCV)对小鼠胶原诱导性关节炎(CIA)的免疫调节作用。方法通过TCV干预CIA模型,观察小鼠发病情况,运用免疫组化技术观察局部关节病变程度;FACs分析T细胞亚群变化;ELISA检测细胞培养上清中TGF-β、IFN-γ、IL-17水平;定量PCR方法检测Treg、Th1、Th17细胞相关转录因子表达水平。结果经过TCV干预,小鼠自身反应性T细胞的增殖能力被显著抑制,CII抗体水平也显著下降;相较CIA组,TCV组Treg细胞百分比上升,Th1、Th17细胞百分比明显降低;各细胞分泌细胞因子的能力也发生相应的变化,其中TGF-β上升,IFN-γ、IL-17降低;Treg细胞转录因子Foxp3基因水平提高,Th1、Th17细胞转录因子T-bet、RORα及RORγt的mRNA水平降低。结论 TCV干预可通过抑制CIA小鼠自身反应性T细胞增殖,上调小鼠体内Treg细胞并抑制Th1、Th17细胞的表达而发挥免疫调节作用,有效降低小鼠发病严重度和关节炎症。
Objective To investigate the immunomodulatory effect of T cell vaccine (TCV) on collagen induced arthritis (CIA) in mice. Methods CIA model was used to observe the incidence of the disease in mice. Immunohistochemistry was used to observe the degree of local joint disease. The changes of T lymphocyte subsets were analyzed by FACS. The levels of TGF-β, IFN-γ, IL-17 Level. Quantitative PCR was used to detect the expression levels of Treg, Th1 and Th17 related transcription factors. Results Compared with CIA group, the percentage of Treg cells in TCV group increased and the percentage of Th1 and Th17 cells in TCV group decreased significantly compared with CIA group after TCV treatment. The proliferation of each cell was significantly inhibited The mRNA and protein levels of T-bet, RORα and RORγt in Th1 and Th17 cells were decreased, while the levels of TGF-β, IFN-γ and IL-17 were also decreased. . Conclusion TCV can inhibit the autoimmune T cell proliferation, upregulate the expression of Th1 and Th17 cells in Treg cells in mice and play an immunomodulatory role, which can effectively reduce the severity of disease and joint inflammation in mice.