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1H-1HCOSY和13C-1HHSQC谱对通过Bio-GelP-2柱(2.5cm×125cm)分离制备的纤维三糖、纤维四糖和纤维五糖进行分析。结果表明,在水溶液中纤维低聚糖还原末端C1位α构型与β构型之比(α/β)随聚合度增大而减小,纤维三糖主要是α构型(α/β,0.52),而纤维四糖和纤维五糖主要是β构型(α/β,分别是0.34和0.25)。X射线光电子能谱(XPS)分析表明纤维低聚糖的C1s主要有2种电子结合能,分别为284.8(C1s1,C—O)和286.1eV(C1s2,O—C—O),C1s1与C1s2之比(C1s1/C1s2)随聚合度增大而增大。
1H-1HCOSY and 13C-1H HSQC spectra were analyzed for cellotriose, cellotetraose and cellooligosaccharide prepared by separation on a Bio-Gel P-2 column (2.5 cm × 125 cm). The results showed that the ratio (α / β) of the C1 configuration α to the β configuration in the reducing end of the oligosaccharide decreased with the increase of the degree of polymerization in the aqueous solution. The cellotriose was mainly in the α configuration (α / β, 0.52), while the cellotetraose and cellobetoses were predominantly in beta configuration (α / β, 0.34 and 0.25, respectively). X-ray photoelectron spectroscopy (XPS) analysis showed that there are two main types of C1s in cellooligosaccharides: 284.8 (C1s1, C-O) and 286.1eV (C1s2, O-C-O) (C1s1 / C1s2) increases with the degree of polymerization.