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目的研究自体骨髓干细胞移植治疗肝硬化患者血清VEGF表达及临床意义。方法对经自体骨髓干细胞移植治疗22例失代偿肝硬化患者,在患者术前以及术后12,4,,8,12周分别抽取静脉血,用ELISA法检测其VEGF含量。结果患者血清VEGF含量在术后开始升高,到第2周达到最高峰(P<0.01),之后逐渐下降,到术后12周时与术前和对照组比较差异已无统计学意义(P>0.05),术后的1,2,48,周分别比对照组要显著升高(P<0.01)。结论血清VEGF水平升高只是为了局部损伤肝组织中血管生成与修复,以改善局部肝纤维化程度,并随干细胞的分化能力减弱而降低,最后恢复到术前水平,因此检测血清VEGF含量对了解自体骨髓干细胞移植后对肝硬化损伤肝组织修复和微环境改善的效果评价有一定的临床意义。
Objective To study the expression of serum VEGF in patients with liver cirrhosis after autologous bone marrow stem cell transplantation and its clinical significance. Methods Twenty-two patients with decompensated cirrhosis were treated with autologous bone marrow stem cell transplantation. Venous blood was collected before and 12, 4, 8 and 12 weeks after operation, respectively. VEGF levels were measured by ELISA. Results The serum level of VEGF in patients began to increase after operation and reached the peak at the second week (P <0.01), and then gradually decreased. There was no significant difference between the two groups at the 12th week after operation (P > 0.05), respectively, after 1, 2, 48, and week respectively than the control group was significantly higher (P <0.01). Conclusions The increase of serum VEGF level is only for local lesion of angiogenesis and repair in liver tissue to improve the degree of local liver fibrosis and decrease with the decrease of differentiation ability of stem cells and finally return to preoperative level. Evaluation of the effect of autologous bone marrow stem cell transplantation on hepatic tissue repair and microenvironment improvement after liver injury has certain clinical significance.