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以同时携带显性核不育基因Ms和显性不育抑制基因Rf的甘蓝型油菜为父本,含有相应隐性等位基因的同类型油菜为母本,经一代杂交成功地把显性核不育基因导入具不同细胞质来源的基因型中。90％以上的杂种一代、回交和复交组合均表现为1可育:1不育的分离。在第一批转育所用的15个亲本中,有3个对由Ms基因控制的雄性不育性有抑制作用,它们能使相应的杂种在F_1全部可育。不同的细胞质对Ms基因的表达没有影响。尽管遗传背景以及异地和不同生长季节种植造成的环境因素的差异对Ms表达有微弱影响,个别组合中有少量半不育株产生,但由Ms基因控制的雄性不育性在绝大多数情形下是稳定的,完全可以应用于甘蓝型油菜的轮回选择研究。
Using the same type of rapeseed containing the recessive allele as the male parent, Brassica napus, which carries both the dominant male sterile gene Ms and the dominant male sterility suppressor Rf, was successfully crossed with the dominant nuclear Infertility genes are introduced into genotypes with different cytoplasmic origins. More than 90% of the hybrid generation, backcrossing and multiple complex showed a fertile: 1 sterile separation. Of the 15 parents used in the first round of transfer, three inhibited male sterility controlled by the Ms gene and enabled the corresponding hybrids to become fully fertile at F_1. Different cytoplasms have no effect on Ms gene expression. Although the genetic background and differences in environmental factors caused by planting in different growing seasons have a weak influence on Ms expression and a few semi-sterile plants are produced in individual combinations, the male sterility controlled by the Ms gene in the vast majority of cases Is stable and can be applied to the study of the cycle selection of Brassica napus.