目的：为了探讨河南省庚型肝炎病毒的感染情况和河南株庚型肝炎病毒基因的变异特征。方法：利用逆转录——巢式——聚合酶链反应检测庚型肝炎病毒ＲＮＡ。将扩增产物克隆入Ｔ载体，进行测序和分析。结果：非甲－非成型肝炎中庚型肝炎病毒ＲＮＡ阳性率为３．３３％（１／３０），在丙型肝炎患者中阳性率为６％（３／５０），献血员中阳性率为０．５％（１／２００），对河南株庚型肝炎病毒ＲＮＡ阳性扩增、克隆、测序，与ＧｅｎＢａｎｋ中ＨＧＶ对应位置序列的同源性为９０％～９８．８％。结论：河南省存在反型肝炎病毒的感染者，ＨＧＶ与ＨＣＶ有重叠感染的现象，献血员中有ＨＧＶ携带者。河南株庚型肝炎病毒部分基因序列与已报道的序列均不完全一致，与国内分离株的同源性高于国外分离株。 Objective: To investigate the infection status of Hepatitis G virus in Henan province and the variation characteristics of Hepatitis G virus in Henan province. Methods: Hepatitis G virus RNA was detected by reverse transcription - nested polymerase chain reaction. The amplified product was cloned into T vector for sequencing and analysis. Results: The positive rate of hepatitis G virus RNA was 3.33% (1/30) in non-A-non-formed hepatitis and 6% (3/50) in hepatitis C patients. The positive rate of hepatitis G virus was 0.5% (1/200). The homology of Henan HGV RNA positive amplification, cloning and sequencing was 90% -98.8% with that of GenBank. Conclusion: There is an infection of HGV and HCV in Hepatitis C virus in Henan Province. There are HGV carriers in blood donors. Henan strains of Hepatitis G virus and some of the sequences reported sequence are not exactly the same, with domestic isolates homology higher than foreign isolates.