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目的探讨长期游泳运动后大鼠大脑额叶运动相关脑区c-fos的表达。方法雄性Sprague-Dawley(SD)大鼠55只,分为对照组(A组,n=5)、持续运动组(B组,n=25)和间歇运动组(C组,n=25),其中B、C组以一定方式进行游泳运动。训练持续6周,B、C组大鼠在末次运动结束后即刻,30min,60min,120min,240min每一时间点随机选取各5只,A组与即刻组同时经戊巴比妥钠腹腔麻醉,依次灌注生理盐水、4%多聚甲醛固定后取脑。行免疫组化,计数c-fos阳性细胞,进行统计学分析。结果c-fos在初级运动皮质(M1)、次级运动皮质(M2)、伏核(A)呈均匀表达,两种游泳运动后,M1区c-fos表达峰值都出现在30min;间歇组M2区c-fos表达峰值在60min,而持续组M2区表达无显著变化;伏核c-fos表达,在间歇组峰值出现在60min,持续组则呈增高趋势,240min仍未见下降。结论游泳运动后不同时刻大鼠脑运动皮质、伏核c-fos的表达可能与运动疲劳、恢复有关。
Objective To investigate the expression of c-fos in the brain related frontal lobes of rats after long-term swimming. Methods Fifty-five male Sprague-Dawley rats were divided into control group (n = 5), continuous exercise group (n = 25) and intermittent exercise group (n = 25) Which B, C group swimming in a certain way. The rats in group B and group C were randomly selected for 5 weeks at each time point immediately after 30min, 60min, 120min and 240min after the last exercise. Rats in group A and group B were simultaneously anesthetized by pentobarbital sodium, Followed by perfusion saline, paraformaldehyde 4% after taking the brain. Immunohistochemistry, count c-fos positive cells for statistical analysis. Results The expression of c-fos was uniform in primary motor cortex (M1), secondary motor cortex (M2) and nucleus accumbens (A). After two swimming exercises, The peak of c-fos expression was 60min, but there was no significant change in persistent M2. The expression of c-fos in nucleus accumbens peaked at 60min in intermittent group and increased in continuous group, but not decreased in 240min. Conclusions The expression of c-fos in cerebral cortex and nucleus accumbens may be associated with fatigue and recovery after swimming.