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以醋栗番茄(‘L03708’)和栽培番茄(‘M82’)为试材,采用15%(w/v)浓度的PEG 6000模拟水分胁迫,研究了干旱敏感性不同的番茄幼苗随水分胁迫时间变化下的失水率、脯氨酸(Pro)含量、丙二醛(MDA)含量、过氧化氢(H2O2)含量以及超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、抗坏血酸过氧化物酶(APX)的活性等指标的变化,以期进一步明确番茄幼苗对水分胁迫的生理效应。结果表明:在水分胁迫下,‘L03708’失水率显著低于‘M82’;在胁迫的1~48h内,‘L03708’的Pro含量显著高于‘M82’;在胁迫1~72h内,‘L03708’的MDA、H2O2含量均显著低于‘M82’,‘L03708’的SOD、POD、CAT、APX活性均高于‘M82’。以上结果表明,番茄幼苗抗旱性强的原因在于能产生更多的渗透调节物质,维持渗透调节能力,增加吸水或保水能力,细胞抗氧化酶系统活性强,清除活性氧等物质,减少细胞膜的氧化反应。
The effects of water stress on tomato seedlings with different drought sensitivities were studied by using PEG 6000 (15% (w / v)) simulated by water stress on cucumber (’L03708’) and cultivated tomato (’M82’ The content of MDA, the content of hydrogen peroxide (H2O2), the activity of superoxide dismutase (SOD), the activity of peroxidase (POD), the content of malondialdehyde (MDA) Catalase (CAT) activity, ascorbate peroxidase (APX) activity and other indicators of changes in order to further clarify the physiological effects of tomato seedlings on water stress. The results showed that under water stress, the water loss rate of ’L03708’ was significantly lower than that of ’M82’; within 1 ~ 48h of stress, the content of Pro of ’L03708’ was significantly higher than that of ’M82’; within 1 ~ 72h of stress, The contents of MDA and H2O2 in L03708 were significantly lower than those in ’M82’. The activities of SOD, POD, CAT and APX in ’L03708’ were higher than those in ’M82’. The above results show that tomato seedlings drought resistance is due to the ability to produce more osmoregulatory substances to maintain osmotic adjustment ability to increase water absorption or water retention capacity of the cell antioxidant enzyme system activity clear active oxygen and other substances to reduce cell membrane oxidation reaction.