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采用聚合酶链反应（ＰＣＲ）和核酸探针杂交技术，检测了１００例临床尖周炎患牙根管内标本中牙龈卟啉菌（Ｐｇ）的分布，并对两种方法进行了对比研究。结果显示：①ＰＣＲ对尖周炎中Ｐｇ的检出率为７４％，核酸探针为７６％，两者的总符合率达到９４％；②在两种方法检测的所有项目对比组之间均未见显著性差异（Ｐ＞０．０５）；③Ｐｇ与尖周炎临床症状中的自发痛、叩痛、臭味和尖周肿胀有关（Ｐ＜０．０１或Ｐ＜０．０５）。提示ＰＣＲ和核酸探针技术均具有准确、快速、方便的优点，适用于对临床采样标本的直接检测
Polymerase chain reaction (PCR) and nucleic acid probe hybridization were used to detect the distribution of Pg in 100 cases of periapical specimens of root canal disease. The two methods were compared. The results showed that: (1) The detection rate of Pg in pericoronitis was 74% and the probe of nucleic acid was 76% in PCR, the total coincidence rate reached 94%; ② There was no significant difference between the two groups (P> 0.05). ③Pg was associated with spontaneous pain, percussion pain, stink and apical swelling in clinical symptoms of periarthritis (P <0.01 or P <0.05). Prompt PCR and nucleic acid probe technology has the advantages of accurate, rapid and convenient for the direct detection of clinical specimens