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AIM:To investigate the relationships between polymorphismsof interleukin-1B(IL-1B)promoter region -511C/T andinterleukin-1 receptor antagonist gene(IL-1RN)andsusceptibility to chronic hepatitis B in Chinese population.METHODS:Genomic DNA was extracted from the peripheralblood of 190 patients with chronic hepatitis B and 249 normalcontrols and then subjected to polymerase chain reaction(PCR)amplification.The PCR products were digested byrestriction endonuclease AvaI.The products of digestion weresubjected to 20 g/L gel electrophoresis and ethidium bromidestaining.RESULTS:The frequencies of IL-1B(-511)genotypes CC,CT and Tr in patients with chronic hepatitis B were 23.7%,49.5% and 26.8%,while 26.1%,47.4% and 26.5%respectively in controls.The results showed that there wasno significant difference in the frequencies of alleles orgenotypes in IL-1B between patients with chronic hepatitisB and controls.The distributions of IL-1B(-511)genotypeCC were significantly different between the two subgroups(HBV-DNA≤1×10~3 copies/mL as subgroup I,HBV-DNA>1×10~3 copies/mL as subgroup Ⅱ)of chronic hepatitis B(P=0.029).Only four of the five kinds of polymorphism(1/1,1/2,2/2 and 1/4)were found in this study.The frequenciesof IL-1RN genotypes 1/1,1/2,2/2 and 1/4 were 88.9%,9.0%,0.5% and 1.6% in patients with chronic hepatitis B respect,while were 81.1%,16.9%,0.4% and 1.6% respectively incontrols.The frequencies of genotypel/2 and IL-1RN allele2 in patients with chronic hepatitis B were lower than thosein controls(P=0.016 and P=0.029,respectively).CONCLUSION:There is an association between polymorphismsof the promoter region -511C/T of IL-1B and IL-1RN intron 2and chronic hepatitis B virus infection.Subjects with IL-1RN2 may be resistant to HBV infection,and IL-1B(-511)genotype CC is closely related with HBV-DNA replication,which gives some new clues to the study of pathogenesis ofchronic hepatitis B.
AIM: To investigate the relationships between polymorphisms of interleukin-1B (IL-1B) promoter region -511C / T andinterleukin-1 receptor antagonist gene (IL-1RN) and susceptibility to chronic hepatitis B in Chinese. METHODS: Genomic DNA was extracted from the peripheralblood of 190 patients with chronic hepatitis B and 249 normal controls and then subjected to polymerase chain reaction (PCR) amplification.The PCR products were digested by restriction endonuclease AvaI.The products of digestion weresubjected to 20 g / L gel electrophoresis and ethidium bromide stabilization .RESULTS: The frequencies of IL-1B (-511) genotypes CC, CT and Tr in patients with chronic hepatitis B were 23.7%, 49.5% and 26.8%, while 26.1%, 47.4% and 26.5% respectively in controls. The results showed that there was no significant difference in the frequencies of alleles or genotypes in IL-1B between patients with chronic hepatitis B and controls. the distributions of IL-1B (-511) genotypeCC were significantly different between the two su bgroups (HBV-DNA≤1 × 10 ~ 3 copies / mL as subgroup I, HBV-DNA> 1 × 10 ~ 3 copies / mL as subgroup Ⅱ) of chronic hepatitis B of polymorphisms (1/1, 1/2, 2 and 1/4) were found in this study. These frequencies of IL-1RN genotypes 1/1, 1/2, 2/2 and 1/4 were 88.9% 9.0%, 0.5% and 1.6% in patients with chronic hepatitis B respect, while others were 81.1%, 16.9%, 0.4% and 1.6% respectively in controls. The frequencies of genotypel / 2 and IL-1RN allele2 in patients with chronic hepatitis B were CONCLUSION: There is an association between polymorphisms of the promoter region -511C / T of IL-1B and IL-1RN intron 2 and chronic hepatitis B virus infection. Articles with IL -1RN2 may be resistant to HBV infection, and IL-1B (-511) genotype CC is closely related with HBV-DNA replication, which gives some new clues to the study of pathogenesis of chronic hepatitis B.