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以苹果10个二倍体和5个三倍体品种为试材,对不同倍性品种产生的花粉粒形状和大小进行了比较。结果表明:(1)倍性间不同形状花粉粒分布明显不同,二倍体品种三角形花粉粒数目多,而三倍体品种三角形花粉粒数目少;三倍体品种圆形及方形花粉粒数目均大幅增多;计算三角形花粉粒数目与圆形花粉粒及方形花粉粒数目之和的比值发现,倍性品种间差异较大,该比值1.2可作为鉴定倍性的临界值。(2)三倍体同一形状花粉粒大小比较分散,二倍体则相对集中,三角形花粉粒中等大小花粉粒数目与大花粉粒及小花粉粒数目之和的比值在倍性间存在明显差异,该比值2.50可作为判别二倍体与三倍体的临界值。利用这两个指标对诱变植株产生的花粉粒形状和大小进行了比较,对孢原组织细胞的倍性水平进行了估计。
Using 10 diploid and 5 triploid varieties of apple as materials, the shape and size of pollen grains produced by different ploidy varieties were compared. The results showed that: (1) The distribution of pollen grains with different shapes was obviously different among the ploidy varieties. The number of triangular pollen grains of diploid varieties was more than the number of triangular pollens of triploid cultivars. The number of round and square pollens of triploid cultivars The ratio of the number of triangular pollen grains to the sum of the number of round pollen grains and square pollen grains was found to be significantly different. The ratio of 1.2 was used as the critical value to identify the ploidy. (2) The size of pollen grains in the same shape of triploid was relatively dispersed, while the diploid was relatively concentrated. The ratio of the number of medium size pollen grains to the sum of the number of large pollen grains and small pollen grains was significantly different between ploidy, The ratio of 2.50 can be used as discriminant diploid and triploid critical value. The shape and size of the pollen grains produced by the mutagenized plants were compared by these two indexes, and the ploidy level of the sporogenous tissue cells was estimated.