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在吸附层析过程中,为使某一化合物正确地进行层析,必须首先选择吸附力合宜的吸附剂,其吸附力通常以活度表示。早在1941年Brockmann等曾用六种极性递增的偶氮染料用小层析管进行氧化铝活度的测定,至今仍广为应用。此法费时长,耗费吸附剂和溶剂均较多。1962年Hermanek等改用薄层法,根据偶氮染料在薄层上的R_f值来决定吸附剂的活度。后法较之前法要简便经济得多,但每次须要计算和核对R_f值。我们的方法,系用小型薄层(12×5×0.08厘米),展开10厘米后,目测偶氮染料之移动距离,用以
In the process of adsorption chromatography, in order for a certain compound to be properly chromatographed, it is necessary to first select an adsorbent with a suitable adsorption force, the adsorption force of which is usually expressed in terms of activity. As early as 1941, Brockmann et al. Have used the determination of the alumina activity in a small chromatographic column with six kinds of azo dyes with increasing polarity and are still widely used. This method takes a long time, consume more adsorbent and solvent. In 1962, Hermanek et al. Switched to the thin layer method, and determined the activity of the adsorbent based on the R_f value of the azo dye on the thin layer. The latter method is much more economical and easier than the previous method, but requires calculating and verifying the R_f value each time. Our method is to use a small thin layer (12 × 5 × 0.08 cm), expand 10 cm, the visual movement of azo dyes to