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目的：制备与分析抗可提取性核抗原（ＥＮＡ）单克隆抗体；方法：以ＥＮＡ为抗原，免疫ＢＡＬＢ／ｃ小鼠，取其脾细胞与小鼠Ｓｐ２／０骨髓瘤细胞融合，经反复筛选和再克隆化，建立能稳定分泌抗ＥＮＡ单克隆抗体（ＭｃＡｂｓ）的杂交瘤细胞株；结果：共获得１１株能稳定分泌抗ＥＮＡ的杂交瘤细胞株。对其中的５种单克隆抗体（ＩＧ５，２Ｅ５，２Ｃ６，４Ｃ１０和４Ｅ６）进行了初步分析，结果如下：小鼠腹水抗体效价范围２×１０－５～３×１０－６；免疫球蛋白亚类鉴定，１株为ＩｇＧ２ａ，１株为ＩｇＧ２ｂ，其余３株均为ＩｇＧ１；结论：ＥＮＡ作为弱免疫原可用于制备单克隆抗体
OBJECTIVE: To prepare and analyze anti-extractable monoclonal antibody (ENA) monoclonal antibody.Methods: BALB / c mice were immunized with ENA as an antigen and their splenocytes were fused with mouse Sp2 / 0 myeloma cells. After repeated screening And then cloned to establish a hybridoma cell line that can stably secrete anti-ENA monoclonal antibodies (McAbs). Results: A total of 11 hybridoma cell lines that can stably secrete anti-ENA were obtained. The five monoclonal antibodies (IG5, 2E5, 2C6, 4C10 and 4E6) were analyzed preliminarily. The results were as follows: the mouse ascites antibody titer ranged from 2 × 10-5 to 3 × 10-6; the immunoglobulin subunit One strain was IgG2a, the other was IgG2b. The other three strains were all IgG1. Conclusion: ENA can be used as a weak immunogen to prepare monoclonal antibodies