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目的探讨小白菊内酯对腹腔注射细菌脂多糖(LPS)致大鼠学习记忆功能减退的改善作用。方法健康成年雄性Wistar大鼠36只,随机分为3组,每组12只。对照组腹腔注射生理盐水,致炎组腹腔注射LPS(10mg/kg),治疗组腹腔注射LPS(10mg/kg)和小白菊内酯(5mg/kg)。依据测试时间不同,每组再分为两个亚组,近期组在LPS注射后第3天开始实验;远期组在LPS注射后第12天开始实验。应用Morris水迷宫实验测试系统,采用定位航行实验和空间探索实验测试大鼠认知功能改变。结果炎症近期,与对照组和治疗组相比,致炎组寻台持续时间显著延长(P<0.05),第二象限停留时间和路程显著缩短,第一次穿越时间显著延长,穿越次数显著减少(P<0.05);炎症远期,3组动物学习记忆能力差异无统计学意义,各组游泳速度差异无统计学意义。结论小白菊内酯可改善腹腔注射LPS近期大鼠学习记忆功能减退。
Objective To investigate the effect of parthenolide on learning and memory abilities of rats induced by intraperitoneal injection of bacterial lipopolysaccharide (LPS). Methods Thirty-six healthy adult male Wistar rats were randomly divided into three groups of 12 rats each. The control group received intraperitoneal injection of normal saline, and the inflammatory group received intraperitoneal injection of LPS (10 mg / kg). The treatment group received intraperitoneal injections of LPS (10 mg / kg) and parthenolide (5 mg / kg). According to the different test time, each group was further divided into two subgroups. The most recent group started the experiment on the third day after LPS injection. The long term group started the experiment on the 12th day after LPS injection. The Morris water maze test system was used to test the changes of cognitive function in rats by using positioning navigation experiment and space exploration experiment. Results Inflammation Recently, compared with the control group and the treatment group, the duration of the seek group in the inflammation group was significantly prolonged (P <0.05), the residence time and distance in the second quadrant were significantly shortened, the first time of crossing was significantly prolonged and the number of crossing times was significantly decreased (P <0.05). In the long-term inflammation, there was no significant difference in learning and memory abilities among the three groups of animals, and there was no significant difference in swimming speed between groups. Conclusion Parthenolide can improve the learning and memory abilities of rats with LPS shortly after intraperitoneal injection.