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目的:研究芒果苷滴丸(MDP)对小鼠实验性肝损伤的保护作用及其机制。方法:以MDP对小鼠灌胃给药,对MDP进行最大耐受量(MTD)测定;采用四氯化碳(CCl_4)、D-氨基半乳糖盐酸盐(D-GalN)诱导小鼠急性肝损伤模型;卡介苗(BCG)加脂多糖(LPS)联合诱导小鼠免疫性肝损伤模型。分光光度法检测血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)含量和肝组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)含量,苏木精-伊红(HE)染色法对肝脏组织作病理切片法检查。结果:①以MDP对小鼠灌胃给药,其MTD为180g·kg~(-1),相当于原药材36g·kg~(-1)。②MDP能显著降低急性、免疫性肝损伤小鼠血清中ALT、AST含量(P<0.01),能降低急性肝损伤小鼠肝匀浆MDA含量(P<0.01),升高肝匀浆SOD、GSH-PX活性(P<0.01),病理结果表明MDP能减轻免疫性小鼠肝损伤的肝损伤程度。结论:MDP对急性、免疫性肝损伤小鼠具有显著保护作用,其作用机制可能与抗脂质过氧化有关。
Objective: To study the protective effect of mangiferin dropping pills (MDP) on experimental liver injury in mice and its mechanism. METHODS: The mice were intragastrically administered with MDP and the maximum tolerated dose (MTD) of MDP was determined. The mice were induced acutely with carbon tetrachloride (CCl_4) and D-galactosamine hydrochloride (D-GalN). Liver injury model; BCG plus lipopolysaccharide (LPS) combined to induce immune hepatic injury model in mice. Spectrophotometric Determination of Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) in Serum and Superoxide Dismutase (SOD), Malondialdehyde (MDA), and Glutathione in Liver Tissue Homogenates Glycopeptide peroxidase (GSH-PX) content, hematoxylin-eosin (HE) staining was used to examine the pathological sections of liver tissue. RESULTS: (1) MDP was administered to mice by intragastric administration. Its MTD was 180 g·kg -1, which was equivalent to 36 g·kg -1 of original drug. 2MDP can significantly reduce the serum levels of ALT and AST in acute and immunological liver injury mice (P<0.01), reduce the liver homogenate MDA content in acute liver injury mice (P<0.01), increase liver homogenate SOD, GSH -PX activity (P<0.01). The pathological results showed that MDP can reduce the degree of hepatic injury in livers of immune mice. Conclusion: MDP has a significant protective effect on acute and immune liver injury mice, and its mechanism of action may be related to anti-lipid peroxidation.