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目的:研究补肾通络方对去卵巢骨质疏松模型大鼠细胞核因子κΒ受体活化因子配体(RANKL)/骨保护素(OPG)基因表达的影响,探讨补肾通络方治疗原发性骨质疏松症的分子机制。方法:通过卵巢去势的方法造成大鼠骨质疏松模型,造模后随机分为6组:假手术组、模型组、仙灵骨葆组(5.0 g.kg-1)、补肾基础组(5.4 g.kg-1)、通络组(0.9 g.kg-1)、补肾通络组(6.3 g.kg-1)。ig给药,1次/d,共10周。治疗10周后,在无菌条件下取出大鼠L3椎体,剔除软组织及骨膜,用逆转录聚合酶链式反应(RT-PCR)检测骨组织RANKL/OPG基因mRNA的表达。结果:与模型组相比,补肾通络组、补肾基础组、通络组RANKL mRNA表达明显下降,RANKL/OPG明显下降(P<0.01),3组间比较,补肾通络组的干预作用明显优于补肾基础组与通络组(P<0.05)。结论:补肾通络方治疗原发性骨质疏松症的作用机制可能与调控RANKL mRNA表达,改善RANKL/OPG,从而抑制破骨细胞活性,降低骨吸收有关。
Objective: To study the effect of Bushen Tongluo Prescription on the gene expression of RANKL / OPG in ovariectomized rats model of osteoporosis, The molecular mechanism of osteoporosis. Methods: Osteoporosis model was induced by ovariectomized rats. After modeling, rats were randomly divided into 6 groups: sham-operation group, model group, Xianlinggubao group (5.0 g.kg- 5.4 g.kg-1), Tongluo group (0.9 g.kg-1), Bushen Tongluo group (6.3 g.kg-1). ig administration, 1 time / d, a total of 10 weeks. After 10 weeks of treatment, rat L3 vertebral bodies were removed under aseptic conditions, and the soft tissues and periosteum were removed. The expression of RANKL / OPG mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results: Compared with the model group, the expression of RANKL mRNA in Bushen Tongluo group, Bushen foundation group and Tongluo group was significantly decreased and RANKL / OPG was significantly decreased (P <0.01). The intervention effect of Bushen Tongluo group was obvious It was better than Bushen foundation group and Tongluo group (P <0.05). Conclusion: The mechanism of Bushen Tongluo Recipe in treating primary osteoporosis may be related to regulating RANKL mRNA expression, improving RANKL / OPG, inhibiting osteoclast activity and decreasing bone resorption.