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利用表型鉴定以及47对均匀分布于水稻12条染色体的SSR标记,对长江上游138个杂交水稻亲本进行检测,并采用遗传相似系数及数学模型分析其群体结构,以了解其遗传多样性信息.结果显示,长江上游杂交水稻亲本的保持系、恢复系以及总体的表型性状遗传变异程度较大,适用于进行遗传多样性分析.利用47对SSR标记共检测到94个等位基因位点,平均每个位点2个;其中有效等位基因数67.05个,占71.33%,Nei氏遗传多样性指数变幅为0-0.51,平均值为0.26.供试材料可分为恢复系类群和保持系类群,与生产上利用的保持系和恢复系高度一致.本研究表明利用SSR标记能详细了解长江上游杂交水稻亲本遗传多样性信息并有效区分恢复系与保持系.
Using phenotypic identification and 47 pairs of SSR markers evenly distributed on 12 chromosomes of rice, 138 hybrid rice parents were detected in the upper reaches of the Yangtze River. Genetic similarity coefficient and mathematical model were used to analyze the population structure to understand the genetic diversity. The results showed that the genetic variation of the maintainer line, restorer line and phenotypic trait of hybrid rice parents in the upper reaches of the Yangtze River was high, which was suitable for genetic diversity analysis. A total of 94 alleles were detected using 47 pairs of SSR markers, With an average of 2 loci per locus. The number of effective alleles was 67.05, accounting for 71.33%, and the Nei’s genetic diversity index was 0-0.51 with an average value of 0.26.The tested materials could be divided into two groups: Which is highly consistent with the maintainer and restorer lines used in production.The present study showed that SSR markers can be used to understand the genetic diversity of parents of hybrid rice in the upper reaches of the Yangtze River and to effectively distinguish between restorer lines and maintainer lines.