【摘 要】
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AIM:To comprehensively identify the proteins of tumor relative antigen C2-Hb3 recognized by colorectal carcinoma monoclonal antibody Hb3.METHODS:Ca-Hb3 was isol
【机 构】
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Cancer Research Institute
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AIM:To comprehensively identify the proteins of tumor relative antigen C2-Hb3 recognized by colorectal carcinoma monoclonal antibody Hb3.METHODS:Ca-Hb3 was isolated by SDS-polyacrylamide gel electrophoresis(PAGE)followed by digestion with trypsin.Trypsin peptides were analyzed by liquid chromatography-tandem mass spectrometry(LC-HS/MS).The proteins identified by mass spectrometry were analyzed using bioinformatics.RESULTS:Ca-Hb3 was identified as a CKAP4-like protein by Nano HPLC tandem mass spectrometry analysis.The molecular weight of CKAP4-like protein was 62.02 kDa,including one hydrophobic region,one transmembrane domain,five coiled coils,four glycosylation sites and forty-nine phosphorylation sites.CKAP4-like protein had a high homogeneity with DeltaNp63a.The characteristic expression of DeltaNp63a that is considered a potential oncogene in the isoforms of p63 was similar to that of Ca-Hb3.CONCLUSION:Ca-Hb3 is probably a CKAP4-like protein,belonging to DeltaNp63a isoform of p63 family.
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