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目的探讨贲门癌变过程中p16基因启动子区甲基化和p16蛋白表达变化特征和规律及其相互关系。方法采用甲基化特异PCR(MSP)及免疫组化方法,检测林州地区32例贲门癌患者癌组织、癌旁不典型增生组织和正常组织p16基因启动子区甲基化状态及蛋白表达情况。结果p16基因在癌组织中表达缺失18例(56%),不典型增生组织中表达缺失8例(73%);26例(81%)癌组织、7例(64%)不典型增生组织和18例(67%)正常组织发生了p16基因启动子区的甲基化。贲门癌组织中p16基因甲基化与表达缺失一致率为56%。差异无统计学意义,P>0.05。结论p16蛋白表达缺失可能是贲门癌变过程中的重要分子事件,p16基因启动子区甲基化可能是导致其蛋白表达缺失的机制之一。
Objective To investigate the characteristics and regularity of methylation and p16 protein expression of p16 gene during cardia carcinogenesis and its relationship. Methods Methylation-specific PCR (MSP) and immunohistochemistry were used to detect the methylation status and protein expression of p16 gene promoter in 32 cases of gastric cardia cancer in Linzhou district. . Results There were 18 cases (56%) in which p16 gene was deleted in cancerous tissues and 8 cases (73%) in atypical hyperplastic tissues. 26 cases (81%) of cancerous tissues and 7 cases (64%) of atypical hyperplasia tissues 18 cases (67%) normal tissue p16 gene promoter region of the methylation. The coincidence rate of methylation and expression of p16 gene in gastric cardia was 56%. The difference was not statistically significant, P> 0.05. Conclusion The loss of p16 protein may be an important molecular event in the process of gastric cardia carcinogenesis. The methylation of p16 gene promoter may be one of the mechanisms leading to the loss of its protein expression.