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目的 探讨肿瘤坏死因子 α(TNF α)对肝星状细胞基质分解素 1及金属蛋白酶组织抑制因子 1(TIMP1)基因表达的影响。方法 在培养的肝星状细胞系中加入 30 μg/LTNF α,于 8、2 4、48、72h 4个不同的时间点收集细胞 ,提取总RNA ;用逆转录定量聚合酶链反应 (PCR)方法测定基质分解素 1及TIMP1的基因表达水平。结果 TNF α组肝星状细胞基质分解素 1基因表达水平在 8、2 4、48、72h 4个时间点均明显高于对照组 ;2 4～ 48h达高峰 ,为对照组的 2倍。TNF α组肝星状细胞TIMP1的基因表达水平在 8、2 4、48h与对照组差异无显著性 (P >0 .0 5 ) ,72h显著升高 ,为对照组的近 3倍。结论 TNF α可增强肝星状细胞基质分解素 1及TIMP1基因的表达。
Objective To investigate the effect of tumor necrosis factor α (TNFα) on the expression of stromelysin1 and tissue inhibitor of metalloproteinase 1 (TIMP1) in hepatic stellate cells. Methods 30 μg / L LTF α was added into cultured hepatic stellate cell line, and the cells were harvested at different time points (8,2,48,72h) to extract total RNA. Reverse transcription quantitative polymerase chain reaction (PCR) Methods The gene expression levels of stromelysin 1 and TIMP1 were determined. Results The expression of stromelysin 1 gene in hepatic stellate cells in TNFα group was significantly higher than that in control group at 8, 2, 4, 48 and 72 hours, reaching the peak at 24-48 hours, which was twice that of control group. The mRNA expression of TIMP1 in TNFα group was not significantly different from that in control group at 8,2,48 and 48 h (P> 0.05), and was significantly increased at 72 h, nearly three times of that in control group. Conclusion TNFα can enhance the expression of stromelysin1 and TIMP1 in hepatic stellate cells.