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目的 探讨癌胚抗原重组痘苗病毒 (CEA rV)对巨噬细胞 (MΦ)抗原呈递功能的影响。方法 采用混合淋巴细胞反应 (MLR) ,检测接种CEA rV小鼠MΦ的抗原呈递功能。采用流式细胞术 (FCM )检测MΦMHC Ⅰ (H 2Kb)、MHC Ⅱ (I Ab)及B7(B7 2 )分子的表达。采用荧光抗体直接染色法 ,检测CEA+肿瘤细胞H 2Kb和I Ab分子的表达。结果 腹腔接种CEA rV小鼠MΦ组 (CEA rVMΦ组 )T细胞分泌IL 2的水平较接种W VV株及NS组显著增强(P <0 .0 1)。小鼠腹腔MΦ上I Ab和B7 2分子的固有表达较低 (分别为 41.7%和 13.7% )。接种W VV株后 ,I Ab 及B7 2分子的表达改变不明显 (分别为 44 .3%和 15 .1% ) ;而接种CEA rV后 ,I Ab及B7 2分子的表达明显增强 (分别为87.2 %和 39.5 % )。各组CEA+肿瘤细胞MHC Ⅰ分子呈强表达 ,但却无MHC Ⅱ分子。结论 腹腔接种CEA rV小鼠的MΦ ,可使MΦ其抗原呈递功能显著增强。MΦ的MHC Ⅱ与B7 2分子表达的增强 ,可能是其抗原呈递功能增强的主要原因 ,提示MHC Ⅱ与B7 2分子可能与CEA rV的抗瘤作用密切相关
Objective To investigate the effect of recombinant vaccinia virus (CEA rV) on the antigen presenting function of macrophages (MΦ). Methods Mixed lymphocyte reaction (MLR) was used to detect the antigen presenting function of MΦ in mice vaccinated with CEA rV. The expression of MΦMHC Ⅰ (H 2Kb), MHC Ⅱ (I Ab) and B7 (B7 2) molecules were detected by flow cytometry (FCM). Fluorescent antibody direct staining was used to detect the expression of H 2Kb and I Ab in CEA + tumor cells. Results The levels of IL-2 secreted by T cells from CEA rVΦ mice treated with intraperitoneal inoculation of CEA rV mice were significantly higher than those from WVV and NS vaccinated groups (P <0.01). Intrinsic expression of I Ab and B7 2 molecules in mouse peritoneal MΦ was lower (41.7% and 13.7%, respectively). After inoculation of WVV strain, the expression of I Ab and B7 2 molecules changed insignificantly (44.3% and 15.1% respectively); while the expression of I Ab and B7 2 molecules was significantly increased after CEV rV vaccination 87.2% and 39.5%). The MHC Ⅰ molecules of CEA + tumor cells in each group were strongly expressed, but no MHC Ⅱ molecules. Conclusion Intraperitoneal inoculation of MΦ in CEA rV mice can enhance the function of MΦ antigen presentation. The enhancement of MHC Ⅱ and B7 2 expression in MΦ may be the main reason for the enhancement of antigen presentation, suggesting that MHC Ⅱ and B7 2 may be closely related to the anti-tumor effect of CEA rV