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目的:探讨胃癌患者外周血和骨髓血中微转移的检测和意义。方法:以CD44v6基因作指标,以半定量RT鄄PCR方法检测46例胃癌患者和6例非恶性病对照者。结果:6例对照者外周血和骨髓血中都未检出CD44v6mRNA表达,46例胃癌患者外周血、骨髓血中CD44v6mRNA的阳性表达率为84.8%和86.9%(39/46例,40/46例),骨髓血阳性表达率稍高于外周血(P>0.05),外周血中弥漫型癌血中32例有30例有CD44v6mRNA表达,表达率93.75%,而肠型癌血中14例仅有9例有表达,表达率64.28%,两者比较差异有显著性;而骨髓血中弥漫型癌32例中31例有CD44v6mRNA表达,表达率96.87%,而肠型癌血中14例仅有10例有表达,表达率为71.42%,两者比较差异有显著性。结论:检测胃癌患者外周血、骨髓血中CD44v6基因的微转移表达可提高胃癌患者临床分期的准确性,并判断预后。
Objective: To investigate the significance and detection of micrometastasis in peripheral blood and bone marrow of gastric cancer patients. Methods: The CD44v6 gene was used as an index to detect 46 gastric cancer patients and 6 non-malignant disease patients by semi-quantitative RT-PCR. Results: No expression of CD44v6 mRNA was detected in peripheral blood and bone marrow of 6 patients. The positive rates of CD44v6 mRNA in peripheral blood and bone marrow of 46 patients with gastric cancer were 84.8% and 86.9% (39/46, 40/46) ), The positive rate of myeloid blood was slightly higher than that of peripheral blood (P> 0.05). There were 30 cases with CD44v6 mRNA expression in 32 cases of diffuse cancerous blood in peripheral blood, the expression rate was 93.75% 9 cases were expressed, the expression rate of 64.28%, the difference between the two was significant; and bone marrow blood in 32 cases of diffuse type of carcinoma in 31 cases of CD44v6 mRNA expression, the rate of 96.87%, while 14 cases of intestinal type of cancer only 10 Cases were expressed, the expression rate was 71.42%, the difference between the two was significant. Conclusion: Detecting the micrometastasis expression of CD44v6 gene in peripheral blood and bone marrow of patients with gastric cancer can improve the clinical staging accuracy and prognosis of patients with gastric cancer.